Effect of polylactic acid-polyethylene glycol composite fibrous materials on adhesion, multiplication and viability of osteoblasts

doi:10.3969/j.issn.2095-4344.2014.39.003

Abstract

Abstract:

BACKGROUND: Recent advances in polylactic acid-polyethylene glycol (PLA-PEG) block copolymer and hydroxyapatite surface-grafted with poly(l-lactide) (HA-g-PLLA), including their synthesis and preparation, have been approved for application in bone tissue engineering. But they have still many disadvantages. Combination of HA-g-PLLA and PEG–PLA could show a great potential for development in bone tissue engineering.
OBJECTIVE: To investigate the effect of PLA-PEG/HA-g-PLLA composite fibrous material with different content of HA-g-PLLA on adhesion, multiplication and cell viability of osteoblasts.
METHODS: Four fibrous materials of 5%PLA-PEG, 5%PLA-PEG/5%HA-g-PLLA, 5%PLA-PEG/10%HA-g-PLLA, 5%PLA-PEG/15%HA-g-PLLA were prepared using electrospinning technique and divided into four experimental groups. Mouse osteoblasts were inoculated on the materials. A statistical analysis was done based on results from cell dyeing and alkaline phosphatase detection at certain time points to evaluate the effect of new materials on osteoblast adhesion, proliferation and viability.
RESULTS AND CONCLUSION: In the experiment of cell multiplication and the test of alkaline phosphatase activity, both absorbance values and activity of alkaline phosphatase from high to low were 5%PLA-PEG/10%HA-g-PLLA > 5%PLA-PEG/15%HA-g-PLLA > 5%PLA-PEG/5%HA-g-PLLA > 5%HA-g-PLLA (P < 0.05). The absorbance value of PLA-PEG/HA-g-PLLA roughly presented a waveform with the increasing addition of HA-g-PLLA. The effect of PLA-PEG/HA-g-PLLA composite fibrous material on adhesion, multiplication and osteogensis of osteoblasts increases with addition of HA-g-PLLA in a certain range.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: tissue engineering, osteoblasts, proliferation, alkaline phosphatase

CLC Number:

R318

Lu Jian, Yang Zhen-lei, Hou Ren. Effect of polylactic acid-polyethylene glycol composite fibrous materials on adhesion, multiplication and viability of osteoblasts [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(39): 6246-6251.

Figures/Tables 3

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